Journal: iScience
Article Title: Dengue virus and Zika virus alter endoplasmic reticulum-mitochondria contact sites to regulate respiration and apoptosis
doi: 10.1016/j.isci.2024.111599
Figure Lengend Snippet: DENV and ZIKV infection decrease the interactions between ERMC proteins (A) Schematic representation of ERMC tethering complexes. Created with BioRender.com . (B and C) Huh7.5 cells were infected with DENV 16681s (MOI = 1) or ZIKV H/PF/2013 (MOI = 10) or left uninfected. Two or three days later, cells were fixed and subjected to proximity ligation assays (PLA) to detect (B) SYNJ2BP-RRBP1 or (C) VAPB-PTPIP51 interactions, and immunostained for NS3 viral protein to identify infected cells. Cells were imaged using confocal microscopy. Representative images are shown. Scale bar = 20 μm. (D) Quantification of PLA dot abundance for RRBP1-SYNJ2BP, VAPB-PTPIP51, and IP3R1-VDAC1 interactions in uninfected- and infected cells from two independent experiments. Values in the 5%–95% percentile range are shown. ∗∗∗∗: p ≤ 0.0001; ∗: p ≤ 0.05; Kruskal-Wallis test for all except for RRBP1-SYNJ2BP 72hpi dataset (normal distribution of the data for the three conditions), which was analyzed with a one-way ANOVA test. Between 40 and 90 cells per condition over 2 independent experiments were analyzed. The “Ab alone” specificity controls were not taken into account for the statistical analysis.
Article Snippet: Rabbit anti-IP3R1 (NBP2-22458) antibodies were obtained from Novus Biologicals.
Techniques: Infection, Ligation, Confocal Microscopy